HPLC Comaprision
| Specifications | Agilent 1220 Infinity Mobile LC Solution | Agilent 1260 Infinity Quaternary LC System | Agilent 1260 Infinity Binary LC System |
|---|---|---|---|
| Key benefits | Dual gradient pump with integrated degasser, 600 bar, up to 10 mL/ min, for reliable and robust analysis Vial sampler with injector programming, expandable to walk-up 80 °C oven for one 25-cm column Variable wavelength or diode array detector with 80 Hz sampling rate for highest sensitivity Mobile Upgrade Kit with attenuation unit for vibration resistance Walk-Up Upgrade Kit with external tray for easy sharing of one instrument for LC/MS analysis | ||
| Pump | |||
| Hydraulic system | Dual plunger in series pump with proprietary servo-controlled variable stroke drive, fl oating plungers and passive inlet valve | Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons | Dual piston in series pump with servo-controlled variable stroke drive, floating pistons |
| Settable fl ow range | 0.001 10 mL/min, in 0.001 mL/ min increments | 0.001 10 mL/min, in 0.001 mL/min increments | Set points 0.001 - 10 mL/min, in 0.001 mL/min increments |
| Flow range | 0.2 10 mL/min | 0.2 10.0 mL/min | 0.2 - 10.0 mL/min |
| Flow precision | <0.07 % RSD, or < 0.02 min SD whatever is greater, based on retention time at constant room temperature | < 0.07 % RSD, or < 0.02 min SD whatever is greater, based on retention time at constant room temperature | <= 0.07 % RSD or <= 0.02 min SD, whatever is greater; based on retention time at constant room temperature |
| Flow accuracy | ± 1 % or 10 ?l/min whatever is greater | ± 1 % or 10 µL/min whatever is greater, pumping degassed H2O at 10 Mpa | ± 1 % or 10 µL/min whatever is greater; pumping degassed H2O at 10 Mpa (100 bar) |
| Pressure Operating range | 0 60 MPa (0 600 bar, 0 8700 psi) up to 5 mL/min Operating range 0 20 MPa (0 200 bar, 0 2950 psi) up to 10 mL/min (all versions) | Operating range 0 60 MPa (0 600 bar, 0 8700 psi) up to 5 mL/min Operating range 0 20 MPa (0 200 bar, 0 2950 psi) up to 10 mL/min | 1260 Infinity Quaternary pump: Operating range up to 60 MPa (600 bar, 8700 psi) up to 5 mL/min | Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 mL/min 1260 Infinity Quaternary pump VL: Operating range up to 40 MPa (400 bar, 5880 psi) up to 5 mL/min | Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 mL/min |
| Pressure pulsation | < 2 % amplitude (typically < 1 %), at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar) | < 2 % amplitude (typically < 1.3 %), or < 3 bar at 1 mL/min isopropanol, at all pressures > 10 bar (147 psi) | < 2 % amplitude (typically < 1.3 %) or < 0.3 MPa (3 bar), whatever is greater, at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi) |
| Recommended pH range | 1.0 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel | 1.0 12.5 , solvents with pH < 2.3 should not contain acids which attack stainless steel | 1.0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel |
| Gradient formation (gradient pump or optional; two solvents) | Low pressure dual mixing/gradient capability using proprietary high-speed proportioning valve Delay volume 600 900 µL, dependent on back pressure | Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve | Low pressure quaternary mixing/gradient capability using high-speed proportioning valve |
| Composition range | 0 95 % or 5 100 %, user selectable | 0 95 % or 5 100 %, user selectable | 0 - 95 % or 5 - 100 %, user selectable |
| Composition precision | < 0.2 % RSD or < 0.04 min SD, whichever is greater, 1 mL/min; based on retention time at constant room temperature | < 0.2 % RSD, or < 0.04 min SD whatever is greater, at 0.2 and 1 mL/min | < 0.2 % RSD or < 0.04 min SD, whatever is greater, at 1 mL/min; based on retention time at constant room temperature |
| Valid Metering Heads | Valid with standard 100 µL metering head installed Valid with standard 900 µL metering head installed | ||
| Pressure Operating range | 0 60 MPa (0 600 bar, 0 8700 psi) | 0 - 60 MPa (0 - 600 bar, 0 - 8850 psi) | 100 µL: Up to 600 bar (60 MPa or 8702 psi) | 900 µL: Up to 200 bar (20 MPa or 2901 psi) |
| Injection range | 0.1 100 ?L in 0.1 ?L increments Up to 1500 ?L with multiple draw (hardware modifi cation required) | 0.1 - 100 µL in 0.1 µL increments (recommended 1 µL increments) Up to 1500 µL with multiple draw (hardware modification required) | 100 µL: 0.1- 100 µL in 0.1 µL increments Up to 1500 µL with multiple draw (hardware modification required) 900 µL: 0.1 - 900 µL in 0.1 µL increments (recommended 1 µL increments) Up to 1800 µL with multiple draw (hardware modification required) |
| Replicate injections | 1 99 from one vial | 1 99 from one vial | |
| Precision | < 0.25 % RSD from 5 100 ?L, < 1% RSD 1 5 ?L variable volume | Typically < 0.25 % RSD of peak areas from 5 - 100 µL, Typically < 1 % RSD of peak areas from 1 - 5 µL, | 100 µL: <0.25% RSD from 5-100 µL, <1% from 1-5 µL, variable volume 900 µL: Typically < 0.5 % RSD of peak areas from 5 - 2000 µL Typically < 1 % RSD of peak areas from 2000 - 5000 µL Typically < 3 % RSD of peak areas from 1 - 5 µL Variable volume |
| Minimum sample volume | 1 ?L from 5 ?L sample in 100 ?L microvial, or 1 ?L from 10 ?L sample in 300 ?L microvial | 1 µL from 5 µL sample in 100 µL microvial, or 1 µL from 10 µL sample in 300 µL microvial | |
| Carryover | Typically < 0.1 %, < 0.05 % with external needle cleaning | Typically < 0.1 %, < 0.05 % with external needle cleaning | 100 µL: Typically < 0.1 %, < 0.05 % with external needle cleaning 900 µL: Typically < 0.1 %, < 0.05 % with external needle cleaning |
| Sample viscosity range | 0.2 50 cp | 0.2 50 cp | 100 µL: 0.2 - 5 cp 900 µL: 0.2 - 5 cp |
| Sample capacity | 100 × 2-mL vials in 1 tray 40 × 2-mL vials in ½ tray 15 × 6-mL vials in ½ tray (Agilent vials only) | 100 × 2 mL vials in 1 tray 40 × 2 mL vials in ½ tray 15 × 6 mL vials in ½ tray (Agilent vials only) | 100 x 2-mL vials in 1 tray 40 x 2-mL vials in 1/2 tray 15 x 6-mL vials in 1/2 tray (Agilent vials only) |
| Injection cycle time | Typically 50 s depending on draw speed and injection volume | 50 s for draw speed 200 µL/min, ejection speed 200 µL/min, injection volume 5 µL | Typically 50 s depending on draw speed and injection volume |
| Column Oven Temperature range | 5 °C above ambient to 80 °C | 10 degrees below ambient to 80 °C up to 80 °C: flow rates up to 5 mL/min | 10 degrees below ambient to 80 °C up to 80 °C: flow rates up to 5 mL/min |
| Column Oven Temperature stability | ± 0.15 °C | ± 0.15 °C | ± 0.15 °C |
| Column Oven Temperature accuracy | ± 0.8 °C with calibration ± 0.5 °C | ± 0.8 °C ± 0.5 °C With calibration | ± 0.8 °C ± 0.5 °C With calibration |
| Column Oven Column capacity | One 25-cm column | Three 30 cm | Three 30 cm |
| Detector type | Variable Wavelength Detector (G4286B, G4288B, G4290B) | Variable Wavelength Detector (VWD)(G1314F) | Variable Wavelength Detector (G1314F) |
| Light source | Deuterium lamp | Deuterium lamp | Deuterium lamp |
| Wavelength range | 190 600 nm | 190 600 nm | |
| Short term noise | ± 0.35 × 10-5 AU, at 230 nm | ± 0.25·10-5 AU at 230 nm | < ± 0.25 x 10 -5 AU, at 230 nm* |
| Drift | 3 × 10-4 AU/hr, at 254 nm | < 1·10-4 AU/h at 230 nm | 1 x 10-4AU/hr, at 230 nm |
| Linearity | > 2 AU (5 %) upper limit | > 2.5 AU (5 %) at 265 nm | >2.5 AU upper limit |
| Wavelength accuracy | ± 1 nm; self-calibration with deuterium lines, verifi cation with holmium oxide fi lter | ± 1 nm | ± 1 nm, self-calibration with deuterium lines, verification with holmium oxide filter |
| Band width | 6.5 nm typical | 6.5 nm typical | 6.5 nm typical over whole wavelength range |
| Diode Array Detector | G4294B only | G4212B | G4212B |
| Detector type | 1024-element diode array | 1024-element photodiode array | 1024-element diode array |
| Light source | Deuterium and tungsten | Deuterium lamp | Deuterium |
| Number of signals | 8 | 8 | |
| Maximum sampling rate | 80 Hz | 80 Hz | 80 Hz (both spectra and signals) |
| Short-term noise | < ± 0.7 x 10-5 AU at 254/4 nm and at 750 nm, TC 2 s | < ± 3 × 10-6 AU at 230 nm/4 nm | < ± 3 x 10-6 AU at 230/4 nm, slit width 4 nm, TC 2 sec, ASTM with 10 mm Max-Light cartridge cell Typically <± 0.6 x 10-6 AU/cm at 230/4 nm, slit width 4 nm, TC 2 sec, ASTM with 60 mm Max-Light cartridge cell |
| Drift | < 0.9 x 10-3 AU/hr at 254 nm | < 0.5 × 10-3 AU/hr at 230 nm | > 2.0 AU (5 %) at 265 nm Typically 2.5 AU (5 %) |
| Linearity | > 2.0 AU (5 %) at 265 nm | > 2.0 AU (5 %) at 265 nm | |
| Wavelength range | 190-950 nm | 190 640 nm | 190-640 nm |
| Wavelength accuracy | ± 1 nm, self-calibration with deuterium lines verifi cation with holmium oxide fi lter | ± 1 nm | ± 1 nm, self-calibration with deuterium lines |
| Slit width | Programmable: 1, 2, 4, 8, 16 nm | 4 nm | 4 nm (fixed) |
| Diode width | < 1 nm | ~ 0.5 nm | ~ 0.5 nm |