Protein expression, purification, and characterization (PEPC10)

This intensive EMBO Practical Course is dedicated to modern methods in sample preparation and characterization of proteins for structural studies. It covers cloning and overexpression in bacterial and eukaryotic systems, protein purification, and sample preparation for small angle X-ray scattering (SAXS) and protein crystallography.

This is a hands-on course for researchers in the early stages of their career who wish to learn molecular biology techniques in sample preparation and characterization for structural studies. It is centered around parallel practicals in small groups with experts using model systems. Participants are also encouraged to bring their own samples for protein crystallization and characterization by thermophoresis and SAXS.